Bone marrow mesenchymal stem cell transplantation suppresses emphysema-induced inflammation and apoptosis
10.3969/j.issn.2095-4344.2014.06.014
- VernacularTitle:骨髓间充质干细胞移植抑制肺气肿炎症反应及细胞凋亡
- Author:
Xiaojian ZHAO
;
Caiping LU
;
Weiwei CHU
;
Qiang ZHEN
;
Guoliang TAN
;
Yaxiao ZHANG
;
Renfeng WANG
;
Bing ZHANG
;
Jiabao LIU
- Publication Type:Journal Article
- Keywords:
stem cells;
mesenchymal stem cells;
bone marrow;
celltransplantation;
pulmonary emphysema;
bronchoalveolar lavage fluid
- From:
Chinese Journal of Tissue Engineering Research
2014;(6):906-911
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bone marrow mesenchymal stem cells transplantation can change the surrounding microenvironment through paracrine mechanisms, and can be employed for treatment of serious damage to lung function through the promotion of angiogenesis, inhibition of apoptosis and maintaining functional stability of autonomic nervous system. OBJECTIVE:To observe the inflammatory reaction in experimental emphysema and inhibition of apoptosis through bone marrow mesenchymal stem cells transplantation.
METHODS:Twenty-four Wistar female rats were randomly divided into three groups:healthy control group, model group and experimental group. In the latter two groups, smoking and endotracheal instil ation of porcine pancreatic elastase were performed to establish emphysema models. After modeling, bone marrow mesenchymal stem cells were injected via tail vein in the experimental group. Pathological changes of the lung, the level of tumor necrosis factor-alpha and cellnumber in the bronchoalveolar lavage fluid as wel as apoptotic index in lveolar wal s were detected after celltransplantation.
RESULTS AND CONCLUSION:In the model and experimental groups, pathological changes of lung tissues were observed to different extent. The lung pathological changes were slighter in the experimental group than the model group (P<0.01). The level of tumor necrosis factor-alpha and apoptotic index in lung tissue were lower in the experimental group than the model group (P<0.01). These findings indicate that bone marrow mesenchymal stem cells can improve emphysema pathological y through inhibition of inflammatory response and apoptosis in experimental emphysema.
