Stromal cell derived factor 1 effects on migration of endogenous neural stem cells
10.3969/j.issn.2095-4344.2014.06.021
- VernacularTitle:基质细胞衍生因子1对内源性神经干细胞的趋化迁移作用
- Author:
Wen SU
;
Peng DING
;
Jinkun WANG
;
Hao ZHANG
;
Linjie MU
;
Bo WANG
;
Jingchuan LIU
;
Guanghui GONG
;
Chongqian WANG
- Publication Type:Journal Article
- Keywords:
neural stem cells;
chemokine CXCL12;
hippocampus;
cellmovement
- From:
Chinese Journal of Tissue Engineering Research
2014;(6):950-955
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Stromal cellderived factor 1 in chemotactic migration of endogenous neural stem cells plays a very important role, but the specific migration mechanism is unclear
OBJECTIVE:To observe the effects of exogenous stromal cellderived factor 1 on chemotactic migration and proliferation of neural stem cells in the rat hippocampus
METHODS:Exogenous stromal cellderived factor 1 (5μL, 500μ/L) was injected into the hippocampus of Sprague-Dawley rats to establish animal models. Brain tissues were taken after days 3, 7, 14 and 21 of perfusion to prepare paraffin sections. Thereafter, nestin expression in the injection region and hippocampus was detected using immunohistochemical method. Experimental control and blank control groups were set.
RESULTS AND CONCLUSION:Paraffin section immunohistochemical results displayed the number of nestin-positive cells in the injection and the hippocampus was gradual y increased. At 3 and 7 days, nestin expression was a little and increased at 14 days, forming a migration tendency to the injection region. At 21 days, there were more nestin-positive cells in the injection area and hippocampus. However, there were no changes as above in the experimental control and blank control groups. The results showed that exogenous stromal cellderived factor 1 may induce the proliferation of neural stem cells in the hippocampus and may be involved in chemotactic migration of endogenous neural stem cells.
