Expression of CD133, CD117, and Ki-67 in human osteosarcoma and their clinical significance
10.3969/j.issn.1000-8179.20131802
- VernacularTitle:骨肉瘤CD133 CD117 Ki-67的表达及与临床病理因素和危险度的相关性研究
- Author:
Jinluan LIN
;
Jianhua LIN
;
Zhaoyang WU
;
Wenbin LAN
;
Xiang LI
;
Weinan LIU
;
Jinyi FENG
;
Fasheng WANG
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
osteosarcoma stem cell;
CD133;
CD117;
Ki-67
- From:
Chinese Journal of Clinical Oncology
2014;(5):305-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to investigate the protein expression of CD133, CD117, and Ki-67 in human osteosarcoma tissues and explore their relationships with the clinico-pathological features and risk of osteosarcoma. Methods:Immunohistochemical method was used to examine the protein expression of CD133, CD117, and Ki-67 gene in the paraffin specimens of 55 and 20 cases of osteosarcoma and osteochondroma, respectively. SPSS17.0 statistical software was used to explore the relationships among the expressions of CD133, CD117, and Ki-67 gene and the biological behavior and prognosis of osteosarcoma. Test criterion:P<0.05 was considered statistically significant. Results:The positive expression rates of CD133, CD117, and Ki-67 were significantly higher in the osteosarcoma tissues than in the benign osteochondroma tissues, and the differences were significant (P=0.016, P=0.008, and P<0.001, respectively). The mean survival and metastasis time were shorter in the CD133 or Ki-67 positively expressed osteosarcoma patients than in those with CD133 or Ki-67 negatively expressed osteosarcoma. The differences were significant (P<0.05). The expression of CD133 and Ki-67, surgical staging, and distal metastasis were relevant to the prognosis of osteosarcoma patients. The expression of CD133 and distal metastasis are the independent risk factors that affect the prognosis of these patients. Conclusion:The positive expressions of CD133 and Ki-67 closely correlated with the occurrence and progression of osteosarcoma, and may be used as an indicator for prognosis of the cancer.
