The inhibitory effects of different curcuminiods onβ-site amyloid precursor protein cleaving enzyme 1 ac- tivity
10.3936/j.issn.1002-0152.2014.01.003
- VernacularTitle:类姜黄素及其单体对β-内分泌酶活性的抑制作用
- Author:
Hongying LIU
;
Zhong LI
;
Bo ZHANG
;
Youfu LI
;
Qiong GU
;
Qingchun GAO
- Publication Type:Journal Article
- Keywords:
Curcumin;
Alzheimer’s disease;
β-amyloid BACE1
- From:
Chinese Journal of Nervous and Mental Diseases
2014;(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective The anti-amyloidogenic effects of Curcumin had been clearly certified, Whether the anti-amyliodogenic effects were mediated through the modulation of BACE1 activity was addressed. Methods SwAPP-HEK293 cells were incubated for 24h without or with 5μM curcumin mix, Cur, DMC and BDMC respectively, then the cells were collected for the cell viability evaluation by using MTT analysis and for the BACE1 activity evaluation by theβ-Secretase Activity Fluorometric Assay Kit. Results The cell viability of swAPPHEK293 cells was unchanged after cur-cuminoids incubation; All of Curcumin mix, Cur, DMC and BDMC could decreased the BACE1 activity, Cur was the most active in suppressing BACE1 activity, and the inhibition strength of others in order was DMC>COM>BDMC. Con-clusion This paper indicate that the anti-amyloidogenic effects of curcumin mix,Cur ,DMC,BDMC may be through the in-hibition of BACE1 activity.
