Experimental study of hypoxia-inducible trophoblastic autophagy involved in the pathogenesis of preeclampsia
10.3969/j.issn.1671-8348.2014.05.022
- VernacularTitle:缺氧诱导滋养细胞自噬参与子痫前期发病的实验研究
- Author:
Hua ZHANG
;
Hongbo QI
- Publication Type:Journal Article
- Keywords:
autophagy;
anoxia;
preeclampsia
- From:
Chongqing Medicine
2014;(5):569-571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study hypoxia environment trophoblast cell autophagy and autophagy of the preeclampsia placental tis-sue .Methods Trophoblastic cell line HTR-8/SVneo were divided into three groups :low oxygen concentration group (group Ⅰand group Ⅱ) and normal oxygen concentration group ;48 h after the application the confocal laser scanning microscopy was used for detection of cytoplasmic autophagosome ,PCR technology analysis of autophagy-related gene expression change of LC3-Ⅱ .LC3-Ⅱ expression levels of 30 cases of preeclampsia placenta were detected by Immunohistochemistry .Results In low oxygen concen-tration group ,there were visible red autophagosome chromatin structure in cytoplasm ,which was extremely rare in the normal oxy-gen concentration group ,in low oxygen concentration group cell LC3-Ⅱ mRNA expression was significantly higher than that of normal concentration group .The preeclampsia placenta of patients with positive immunostaining of LC 3-Ⅱ was 67 .12% ,compared with 9 .14% in the normal control ,there was a significant difference between two groups .Conclusion Preeclampsia placenta auto-phagy activity increased ,hypoxia can induce autophagy trophoblast cell line phenomenon .
