Inhibitory effects of flufenamic acid on gap junction of vascular smooth muscle cells in guinea pig

VernacularTitle:氟灭酸对豚鼠微动脉平滑肌细胞间缝隙连接的抑制作用
Author: Zhiping ZHANG ; Junqiang SI ; Xinzhi LI ; Li LI ; Lei ZHAO ; Lili WEI ; Xiushi YU ; Ketao MA
Publication Type:Journal Article
Keywords: patch-clamp techniques; myocytes,smooth muscle; arteriole; flufenamic acid; gap junction
From: Chongqing Medicine 2014;(5):572-574
CountryChina
Language:Chinese
Abstract: Objective To investigate the influence of flufenamic acid (FFA) on gap junction intercellular communication in vascu-lar smooth muscle cells(VSMC) in situ of acutely isolated arteriole segments .Methods Whole-cell patch clamp recordings were used to study the effects of FFA on membrane input capacitance (Cinput ) ,membrane input conductance(Ginput ) or membrane input re-sistance(Rinput ) of VSMCs embedded in arteriole segments .Results FFA concentration-dependently and reversibly suppressed Ginput or increased Rinput ,with an IC50 of 56 and 33μmol/L in acutely isolated mesenteric artery(MA) and brain artery(BA) segments re-spectively .There was not significant difference between MA and BA (P> 0 .05) .After application of FFA (≥ 300 μmol/L) ,the Cinput ,Ginput and Rinput of the in situ cells were very close to the respective dispersed cell in MA and BA .Conclusion FFA is a reversi-ble gap junction blocker ,achieving a complete electrical isolation of the recorded VSMC at ≥300 μmol/L .FFA suggesting a homo-geneous property of the gap junctions between MA and BA .