Expression and significance of TLR4 and NF-κB on inflammatory injure after intracerebral hemorrhage in rats
10.3969/j.issn.1671-8348.2014.05.027
- VernacularTitle:TLR4和 NF-κB在脑出血大鼠炎症损伤中的表达及意义
- Author:
Shasha YANG
;
Qingyou TIAN
;
Hongxia ZHOU
;
Lingli MENG
;
Zuofeng ZHANG
;
Qian WANG
;
Zifeng WEI
- Publication Type:Journal Article
- Keywords:
intracerebral hemorrhage;
Toll-like receptor 4;
NF-κappa B;
inflammatory
- From:
Chongqing Medicine
2014;(5):584-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate expression and significance of TLR4 and NF-κB on inflammatory injure after intracerebral hemorrhage in rats .Methods 60 Sprague Dawley maleness rats were randomly divided into Sham group ,12 h ,24 h ,72 h and 7 d af-ter ICH group(12 s) .The ICH was induced by injection of autologous blood in rats .The behavioral changes were detected by neu-rologic deficit score .The water content of the brain was used to evaluate brain edema changes .Number of TLR4 and NF-κB positive cells by Nissl staining and the expression of protein determined by immunohistochemistry and Western blot .Results After ICH 12 h ,expression of TLR4 and NF-κB positive cells around the hematoma were expressed ,with the extension of the time ,expression was gradually increasing ,and after ICH 72 h the expression of protein were the highest .Cerebral edema and severe neurological damage occurred .Western blot shows the amount of TLR4 expression and NF-κB were in line with the result .Conclusion After in-tracerebral hemorrhage in rat causing inflammatory injure of brain tissue around the hematoma .TLR4 may activate the expression of NF-κB involved in the secondary inflammatory injure after intracerebral hemorrhage in rats .
