Influence and significance of DHA on expression of angiogene-sis-related genes in SGC7901 cells
10.3969/j.issn.1000-8179.20131578
- VernacularTitle:二氢青蒿素对胃癌细胞血管生成及相关基因表达的影响
- Author:
Aijun WANG
;
Hua SHI
;
Baojun ZHENG
;
Junwei FENG
;
Hongyu WANG
;
Xiao WU
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
artemisinins;
angiogenesis
- From:
Chinese Journal of Clinical Oncology
2014;46(4):227-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influence and significance of DHA on expression of angiogenesis-related genes in SGC7901 cells. Methods:SGC7901 were treated with DHA (5, 10, 20, 40, and 80μmol/l) for different times (24, 48, and 72 h), and the growth inhibition was detected by MTT. The expression of vascular endothelial growth factor (VEGF-C), cyclooxygenase-2 (COX-2) vascular cell adhesion molecule-1 (VCAM-1), and PTEN mRNA were detected by fluorescence-based quantitative poly-merase chain reaction (qRT-PCR). Their corresponding protein levels were tested by Western blot. Results:DHA significantly inhibited the growth of SGC7901 cells in a dose-and time-dependent manner (P<0.05). The expression of the angiogenesis-related genes signifi-cantly changed, as shown by RT-PCR and Western blot analyses. Compared with the control group, the expressions of VEGF-C, COX-2, and VCAM-1 were down-regulated, whereas the expressions of PTEN were up-regulated, after DHA treatment (P<0.05). Con-clusion:DHA inhibits cell growth in gastric cancer SGC7901 cells. The effect may be due to its reduction of VEGF-C, COX-2, and VCAM-1 gene expression, as well as its promotion of PTEN expression in gastric cancer cells.
