Determination of Triphenylmethane Dye Residues in Water by Ultra Performance Liquid Chromatography-Triple Quadrupole Linear Ion Trap Mass Spectrometry
10.3724/SP.J.1096.2014.30732
- VernacularTitle:超高效液相色谱-三重四极杆/复合线性离子阱质谱测定水样中三苯甲烷类及代谢物
- Author:
Yan ZHOU
;
Yonggang ZHAO
;
Beibei ZHANG
;
Yong ZHANG
;
Guosong CHEN
- Publication Type:Journal Article
- Keywords:
Triphenylmethane dye residues;
Ultra performance liquid chromatography;
Triple quadrupole linear ion trap of mass spectrometry;
Solid phase extraction;
Water sample
- From:
Chinese Journal of Analytical Chemistry
2014;(3):367-374
- CountryChina
- Language:Chinese
-
Abstract:
A method for the analysis of five types of triphenylmethane dye residues in water was developed by using solid phase extraction ( SPE) combined with ultra performance liquid chromatography coupled to triple quadrupole linear ion trap mass spectrometry ( UPLC-MS/MS/MS ) . The water samples were extracted and cleaned with mixed-mode cation exchange solid-phase extraction cartridges ( MCX) . The UPLC separation was performed on a C18 column with a linear gradient elution program of acetonitrile and 5 mmol/L aqueous ammonium acetate ( containing 0. 02% formic acid) as mobile phase. Triphenylmethane dye residues were analyzed by mass spectrometry under an electrospray ionization interface ( ESI ) in positive ion mode with MRM-IDA-EPI mode. The calibration curves of five types of triphenylmethane dyes were linear in the range of 0. 02-20 μg/L, and the correlation coefficients were not less than 0. 998. The recoveries at spiked levels of 5 , 10 and 20 μg/L were in the range of 70 . 9%-101%, and the RSDs were 3 . 4%-11 . 9% ( n=6 ) . The limit of detection and quantification were 0. 42-2. 18 ng/L and 1. 68-8. 73 ng/L, respectively. The method was performed as sensitive and accurate, and also suitable for simultaneous determination of five types of triphenylmethane dye residues in water.
