Turn-on Fluorescent Probe Based on Quantum Dots for Detection of Trace Amount Glutamate Dehydrogenase
10.3724/SP.J.1096.2014.30646
- VernacularTitle:荧光增强型量子点探针检测痕量谷氨酸脱氢酶
- Author:
Min YANG
;
Tao YU
;
Zhongping ZHANG
;
Suhua WANG
;
Hui JIANG
- Publication Type:Journal Article
- Keywords:
Fluorescence analysis;
Quantum dot;
Glutamate dehydrogenase;
Nicotinamide adenine dinucleotide;
Liver damage
- From:
Chinese Journal of Analytical Chemistry
2014;(3):436-440
- CountryChina
- Language:Chinese
-
Abstract:
We reported a simple and fast fluorescence system based on quantum dots ( QDs ) to detect glutamate dehydrogenase ( GLDH) , which inverted glutamate to α-ketogrutarate using NAD+ as a coenzyme. The fluorescence of CdTe QDs was quenched by nicotinamide adenine dimucleotide ( NAD+) through an electron transfer pathway, and the quencher NAD+ could be consumed by adding NAD+-dependent enzymes and corresponding substrates. Based on this principle we introduced GLDH to consume NAD+ in the QDs/NAD+ system, leading to the enhancement of the fluorescence intensity of QDs, which was in proportional to the amounts of GLDH added. Using this fluorescence system, we measured GLDH in a wide concentration range from 10 U/L to 1000 U/L, which was of significance in clinical diagnosis of different kinds of liver diseases.
