Double-chamber stirred bioreactor improves the repaired effect of beta-tricalcium phosphate on goat knee cartilage defects
10.3969/j.issn.2095-4344.2014.03.008
- VernacularTitle:双腔搅拌式生物反应器提高β-磷酸三钙修复关节软骨缺损的效果
- Author:
Jianwei LI
;
Hui ZHAO
;
Xiaoqiang ZHANG
;
Lei WANG
;
Liheng XIA
;
Dan JIN
;
Gang WANG
;
Bin YU
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
tissue engineering;
bioreactors;
stem cells;
compomers
- From:
Chinese Journal of Tissue Engineering Research
2014;(3):377-382
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:A preliminary experiment developed a double-chamber stirred bioreactor which can carry out osteogenic and cartilage induction at the same time.
OBJECTIVE:To explore the effects of double-chamber stirred bioreactor on the repair of goat knee cartilage defects with tissue-engineered cartilage.
METHODS:Twelve goats were selected to make bilateral femoral condyle osteochondral defects models and randomized to three groups:experimental group, implanted with the composites ofβ-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor under mechanical stimulation;control group, implanted with the composites ofβ-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor;blank control group, without treatment. After 12 and 24 weeks of implantation, general observation, Masson staining, II col agen immunohistochemical staining and histological scoring were performed.
RESULTS AND CONCLUSION:In the experimental and control groups, new cartilage tissue and bone tissue were visible, but the experimental group showed better repair effects than the control group (P<0.05). The blank control group had no cartilage formation. These findings indicate that under the mechanical stimulation by the double-chamber stirred bioreactor in vitro, the repair effect of tissue-engineered osteochondral complex on knee joint cartilage defects can be improved.
