Cartilage surface of early osteoarthritis in rats induced by papain under scanning electron microscope
10.3969/j.issn.2095-4344.2014.02.003
- VernacularTitle:木瓜蛋白酶诱导大鼠膝早期骨关节炎软骨表面的电镜扫描
- Author:
Zongbao WANG
;
Yalin XU
;
Weiming LIAO
;
Qingyou LU
;
Zhaohui CHEN
;
Xiangyang WU
;
Xu SUN
;
Wenxiu DUAN
;
Yongjun BAO
;
Yuelan DAI
- Publication Type:Journal Article
- Keywords:
osteoarthritis;
papain;
L-cysteine;
cartilage,articular
- From:
Chinese Journal of Tissue Engineering Research
2014;(2):177-182
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Osteoarthritis animal models induced by papain are commonly used for studying osteoarthritis.
OBJECTIVE:To observe the morphology of articular cartilage surface during early osteoarthritis process in rats induced by papain and L-cysteine under scanning electron microscope.
METHODS:The right knees of Sprague-Dawley rats were given 0.15 mL mixed solution of 2%papain and 0.03 mol/L L-cysteine in the ratio of 2:1. The left knees were given injections of 0.15 mL 0.9%NaCl, as the control group. Another two rats (four knees) maintained untreated, as the normal control group. Al the rats were observed with scanning electron microscope at 2, 4, 6 weeks after injection, to explore the surface morphology of medial condyle of femur.
RESULTS AND CONCLUSION:The condyles of normal and control groups revealed numerous shal ow pits on the surface of articular cartilage. At 2 weeks after injection, the surfaces of condyles were unsmooth, presenting the shrinkage and distortion. The thinner articular cartilage and local tiny cracks were seen at 4 weeks. The deep and great cracks and cartilage defect were observed on the surface of cartilage at 6 weeks. The time of 4-6 weeks is a reference point for establishing early osteoarthritis model in rats through injections of 2%papain and 0.03 mol/L L-cysteine.
