Odontogenesis-related gene expression during in vitro culture of tooth germ cells
10.3969/j.issn.2095-4344.2014.02.006
- VernacularTitle:牙胚细胞体外培养过程中成牙基因的表达
- Author:
Jiaojiao BA
;
Huiyu HE
;
Yang HU
;
Mei MA
;
Xiangzhen HAN
- Publication Type:Journal Article
- Keywords:
cell culture techniques;
tooth germ;
gene expression;
odontogenesis
- From:
Chinese Journal of Tissue Engineering Research
2014;(2):193-198
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Some studies have indicated that different genes in tooth germ tissue play a role at different time, contributing to tooth germ development.
OBJECTIVE:To observe the expressions of dentin matrix protein 1, enamel protein, col agen I and homeobox gene 1 at different stage of in vitro culture of tooth germ cells.
METHODS:RNA from tooth germ cells was extracted at days 1, 3, 6 of in vitro culture. After reverse transcription, real-time quantitative PCR detection was adopted to measure relative expression of dentin matrix protein 1, enamel protein, col agen I and homeobox gene 1 mRNA.
RESULTS AND CONCLUSION:Dentin matrix protein 1, enamel protein, and col agen I mRNA expressions increased with culture time, and reached the peak at day 3 (P<0.05), whereas homeobox gene 1 mRNA decreased with culture time (P<0.05).
