Improved suture occlusion method to prepare focal cerebral ischemia reperfusion models in Sprague-Dawley rats
10.3969/j.issn.2095-4344.2014.02.011
- VernacularTitle:改良线栓法制作SD大鼠局灶性脑缺血再灌注模型
- Author:
Nianxia SUN
;
Weijuan GAO
;
Zhongliang YI
- Publication Type:Journal Article
- Keywords:
models,animal;
reperfusion injury;
carotid artery,external;
rats,Sprague-Dawley
- From:
Chinese Journal of Tissue Engineering Research
2014;(2):225-230
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:During the progress of focal cerebral ischemia-reperfusion in rat with transient suture occlusion, bleeding combating and the line being plugged into the cerebral middle artery smoothly are important for successful modeling and research focus.
OBJECTIVE:To improve Longa suture occlusion method for focal cerebral ischemia reperfusion model in Sprague-Dawley rats in order to establish a simple surgical procedure with low rate of intraoperative bleeding and high rate of successful models.
METHODS:The two ends of the external carotid artery were clipped with electric knife, in addition to ligation of surgery lines intraoperatively;with the help of pincett, a fishing line was plugged into the carotid artery along the anteriomedialis artery wal (the direction of the internal carotid artery from the pterygopalatine arterial bifurcation). Postoperative behavior scores in rats, infarct volume calculation and histopathological evaluation under optical microscope were done.
RESULTS AND CONCLUSION:Transverse sections of the external carotid artery were changed from a“O”
shape to a“-”shape after being clipped with electric knife. Subsequently, line nodes were not easy to fal off any more, effectively preventing intraoperative bleeding. With the power of pincett, the rate of plugging the fishing line into artery was significantly increased. Rat’s neurological score and infarct volumes were significantly increased, and brain tissue pathological injury worsened. The modified suture occlusion method for focal cerebral ischemia reperfusion models effectively prevented line nodes off, successful y increased the rate of suture occlusion and models in Sprague-Dawley rats were developed successful y.
