Preparation of RuBpy-doped Silica Fluorescent Nanoprobes and Their Applications to Recognition of Liver Cancer Cells
10.3724/SP.J.1096.2014.30849
- VernacularTitle:包裹RuBpy二氧化硅荧光纳米探针的制备及其用于肝癌细胞的识别
- Author:
Minyan CHEN
;
Zezhong CHEN
;
Wei WANG
;
Lian ZHU
;
Hongwu TANG
;
Daiwen PANG
- Publication Type:Journal Article
- Keywords:
Dye-doped silica nanopartilcles;
Bioconjugation;
Liver cancer cells;
Carcinoembryonic antigen
- From:
Chinese Journal of Analytical Chemistry
2014;(3):326-331
- CountryChina
- Language:Chinese
-
Abstract:
Two kinds of different functional groups modified RuBpy-doped silica fluorescent nanoprobes Probe A and B that conjugated with avidin were prepared for the recognition of liver cancer cells. Firstly RuBpy-doped silica nanoparticles were synthesized by reverse microemulsion and modified with different functional groups, then Probe A was prepared by the conjugation of avidin with carboxyl modified nanoparticles through covalent binding using 1-ethyl-3-( 3-dimethylamino propyl ) carbodiimide hydrochloride ( EDC )/sulfo-NHS, whereas Probe B was prepared by the conjugation of avidin with the polyethylene glycol ( PEG) linkers on the surface nanoparticles using cyanogen bromide method. Therefore, compared with Probe A, Probe B was obtained by coupling avidin to the nanoparticles through long-chain PEG molecules. The two probes were incubated with liver cancer cells respectively, and microscopic fluorescence imaging shows that Probe B which contained PEG molecules could be more effectively applied for the recognition of tumor marker carcinoembryonic antigen ( CEA) in liver cancer cells.
