Effect of tissue factor pathway inhibitor-2 on the expressions of matrix metalloproteinase 1 and 2 in keratocytes
10.3969/j.issn.2095-4344.2014.02.015
- VernacularTitle:角膜基质细胞基质金属蛋白酶1，2活性与组织因子途径抑制物2的效应
- Author:
Jianxiong YU
;
Jing YUAN
;
Lianhong ZHOU
- Publication Type:Journal Article
- Keywords:
corneal stroma;
metalloproteinase;
extracellular matrix;
cornea;
culture medium
- From:
Chinese Journal of Tissue Engineering Research
2014;(2):251-258
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The degradation of extracellular matrix, which is mediated by matrix metal oproteinases, plays a crucial role in the corneal neovascularization. Tissue factor pathway inhibitor 2, a new type serine proteinase inhibitor, can effectively inhibit the activity of matrix metal oproteinases.
OBJECTIVE:To elucidate the effect of tissue factor pathway inhibitor 2 on the expressions of matrix metal oproteinases in keratocytes in vitro.
METHODS:Rabbit keratocytes were primarily cultured and subcultured in vitro. Plasmid vector pBos-Cite-neo/TFPI-2 was transfected into keratocytes with Lipofectamine 2000. The positive cells were selected using G418.
RESULTS AND CONCLUSION:The results of reverse transcription-polymerase chain reaction, western blot analysis and gelatinase zymography analysis showed that, expression of mRNA and protein of tissue factor pathway inhibitor 2 was upregulated in the transfected keratocytes (P<0.05), while activity of matrix metal oproteinase 1 and 2 was significantly decreased (P<0.05). Experimental findings indicate that that tissue factor pathway inhibitor 2 strongly inhibits the activity of matrix metal oproteinase 1 and 2 in keratocytes.
