Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Coccidiostats in Chicken Skin and Fat
- VernacularTitle:超高效液相色谱-串联质谱法测定鸡皮及脂肪组织中抗球虫类药物残留
- Author:
Yingyu WANG
;
Xi XIA
;
Xiaowei LI
;
Shuangyang DING
;
Jianzhong SHEN
- Publication Type:Journal Article
- Keywords:
Ultra performance liquid chromatography;
Tandem mass spectrometry;
Coccidiostats;
Residue
- From:
Chinese Journal of Analytical Chemistry
2014;(3):409-414
- CountryChina
- Language:Chinese
-
Abstract:
A method based on ultra performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS) has been proposed for the determination of coccidiostat residues in chicken skin and fat. The sample was extracted with the combination of methanol, acetonitrile, and acetic acid, and cleaned-up by Sep-Pak tC18 solid phase extraction cartridge. Data acquisition under positive electrospray mode was performed by applying multiple reaction monitoring for both identification and quantification. The limits of detection and quantification for halofuginone and robenidine were 7 μg/kg and 20 μg/kg, respectively. The limit of detection of salinomycin, monensin, narasin, maduramicin, and lasalocid was 5 μg/kg, and limit of quantification was 15 μg/kg. The recovery was 75% to 110% in the spiked concentration range from 15 μg/kg to 200 μg/kg, with intra-day precision lower than 12. 8%, and inter-day precision lower than 13 . 4%.
