Biocompatibility of hydroxyapatite/TiO2 nanotube composites
10.3969/j.issn.2095-4344.2014.03.002
- VernacularTitle:羟基磷灰石/TiO2纳米管复合物的生物相容性
- Author:
Hangzhou ZHANG
;
Yu SUN
;
Lin WANG
;
Ang TIAN
;
Xiangxin XUE
;
Xizhuang BAI
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
nanoparticles;
nanotubes;
durapatit;
materials testing
- From:
Chinese Journal of Tissue Engineering Research
2014;(3):335-340
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Hydroxyapatite has excellent biocompatibility, but biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites is rarely reported.
OBJECTIVE:To evaluate the biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites.
METHODS:First, the TiO2 nanotubes were fabricated on the surface of the titanium by anodic oxidation
technique. Second, the nano-hydroxyapatite/TiO2 nanotube composites were fabricated by electrodeposition technique. The surface morphology of the composites was observed by scanning electron microscopy. Mouse osteoblasts MC-3T3-E1 were co-cultured with the nano-hydroxyapatite/TiO2 nanotube composites, TiO2 nanotubes and titanium, and commercial y pure titanium to observe the celladhesion, proliferation and necrosis on scaffolds.
RESULTS AND CONCLUSION:The morphology of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites could be control ed by altering the conditions of the anodic oxidation and electrodeposition. Under the inverted microscope, after 3 days of co-culture with TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites, MC-3T3-E1 cells proliferated wel with regular shape and arrangement that were superior to those on commercial y pure titanium. Under scanning electron microscope, the cellwere adhered and proliferated wel on the surface of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites after 3 days. Apoptosis rate of the cells was significantly reduced on the surface of nano-hydroxyapatite/TiO2 nanotube composites (7.8%) compared with TiO2 nanotubes (9.4%) and commercial y pure titanium (13.5%), indicating nano-hydroxyapatite/TiO2 nanotube composites have good biocompatibility.
