Effect of Effective Monomer of Kangxianling Prescription on Extracellular Matrix Components of 5/6 Nephrectomy Rat
10.3969/j.issn.1005-5304.2014.03.013
- VernacularTitle:抗纤灵方有效单体对5/6肾切除大鼠细胞外基质成分的影响
- Author:
Ying TANG
;
Yi ZHU
;
Liqun HE
;
Dong WANG
;
Tingting XIE
- Publication Type:Journal Article
- Keywords:
Kangxianling prescription;
renal fibrosis;
Chinese medicine monomer;
LN;
FN;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(3):43-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of effective monomer of Kangxianling prescription on renal function and extracellular matrix composition of 5/6 nephrectomy rats model, and provide basis for the screening of effective traditional Chinese medicine of anti-renal fibrosis. Methods Sixty SD rats were randomly divided into normal group, model group, chrysophanol group, salvianolate A group, oleanolic acid group and losartan group. The kidney fibrosis model was made by operation. Two months after intervented by correspong drugs, renal pathological changes of all groups were observed, the levels of renal function indexes were detected, and real-time PCR assay was used to detect laminin (LN), fibronectin (FN), type Ⅲ collagen (C-Ⅲ), type Ⅰ collagen (C-Ⅰ) mRNA expression in rat kidney tissue. Results SCr and BUN in model group increased significantly compared with the normal group (P<0.01). SCr and BUN in salvianolate A group decreased significantly compared with the model group (P<0.05, P<0.01). BUN in salvianolate A group decreased significantly compared with the losartan group (P<0.01). The expression of C- ,Ⅲ C-Ⅰand LN were reduced by effective monomer of Kangxianling prescription. Conclusion Effective monomer of Kangqianling prescription can inhibit renal fibrosis through reducing the expression of extracellular matrix components, thereby improve the renal function.
