Determination of Polysaccharides in Weixuening Granule by Improved Differential Phenol-sulfuric Method
10.3969/j.issn.1005-5304.2014.03.025
- VernacularTitle:改进差示苯酚-硫酸法测定维血宁颗粒多糖含量
- Author:
Hui CHEN
;
Zhipan YAN
;
Fangyuan CHEN
;
Jilong WANG
;
Shuchang WEI
;
Bihui WANG
- Publication Type:Journal Article
- Keywords:
Weixuening Granule;
phenol-sulfuric method;
polysaccharides;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(3):84-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of polysaccharides in Weixuening Granule, which can be used for quality control of the preparation. Methods Polysaccharides was determined by improved differential phenol-sulfuric method with glucose as reference, and the color conditions were optimized at the wavelength of 487 nm, with the concentration of phenol and sulfuric acid, and reaction time as investigated factors. Results The best color conditions of polysaccharides were 0.04 g/mL phenol 1 mL, 87.5% sulfuric acid 5 mL, and reaction time of 15 min. Glucose had a good linear relationship with absorbanceΔA at the range of 15.3-306.0μg, r=0.999 7. The average recovery was 103.5%, and RSD was 2.9%. The average content of polysaccharides in Weixuening Granule was 27.04%and RSD was 1.1%. Conclusion The method is simple, accurate, stable and reliable, and can be used to determine the content of polysaccharides in Weixuening Granule.
