Low-dose reduced glutathione promotes umbilical cord blood mesenchymal stem cell proliferation
10.3969/j.issn.2095-4344.2014.01.011
- VernacularTitle:低剂量还原型谷胱甘肽可促进人脐血干细胞增殖
- Author:
Xin QU
;
Xu DI
;
Lu HAN
;
Haichao ZHANG
- Publication Type:Journal Article
- Keywords:
stem cells;
umbilical cord;
mesenchymal stem cells;
glutathione
- From:
Chinese Journal of Tissue Engineering Research
2014;(1):63-68
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:How to obtain a sufficient number of cells is one of the key issues in the celltransplantation therapy, and studies have shown that stem cellproliferation can be promoted by reasonable stimulus.
OBJECTIVE:To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells.
METHODS:The cells were divided into two groups:the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione.
RESULTS AND CONCLUSION:At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P<0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P<0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of human umbilical cord blood mesenchymal stem cells.
