Induced differentiation of human umbilical cord mesenchymal stem cells into neural stem cells in vitro
10.3969/j.issn.2095-4344.2014.01.013
- VernacularTitle:体外诱导人脐带间充质干细胞向神经干细胞的分化
- Author:
Weiwei LI
;
Xingyu YAO
;
Limin YANG
;
Pengwei ZHAO
;
Guohua ZHANG
- Publication Type:Journal Article
- Keywords:
stem cells;
mesenchymal stem cells;
umbilical cord;
neural stem cells;
epidermal growth factor;
fibroblast growth factors
- From:
Chinese Journal of Tissue Engineering Research
2014;(1):75-80
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Compared with mesenchymal stem cells from bone marrow and fat, human umbilical cord-derived mesenchymal stem cells, as one of the most potential cellsources to repair the central nervous system, are easy-based, more primitive, and not limited by ethical and legal.
OBJECTIVE:To explore differentiation of human umbilical cord-derived mesenchymal stem cells into neural stem cells induced by the recombinant human basic fibroblast growth factor and recombinant human epidermal growth factor.
METHODS:The human umbilical cord-derived mesenchymal stem cells were induced by basic fibroblast growth factor and epidermal growth factor in vitro. The cellmorphology was observed by invert microscope. The differentiation status was detected by immunofluorescence. The Nestin expression in mRNA level before and after induction was detected by real-time PCR.
RESULTS AND CONCLUSION:The neural stem cellbal s were observed after induction. And the Nestin was detected by immunofluorescence and real-time PCR. Nestin could further differentiate to the neuronal markproteins neuron-specific enolase, microtubule-associated protein 2 protein and glial fibril ary acidic protein. Results from this study show that basic fibroblast growth factor and epidermal growth factor can induce human umbilical cord-derived mesenchymal stem cells to differentiate to neural stem cells, neurons and glial cells.
