Transplantation of bone marrow mesenchymal stem cells affects the proliferation and function of CD4+T cells in mice
10.3969/j.issn.2095-4344.2014.01.018
- VernacularTitle:骨髓间充质干细胞移植小鼠免疫系统CD4+T细胞亚群的变化
- Author:
Shaohong SU
;
Junfeng ZHANG
;
Qianru LI
;
Shasha GUAN
;
Ying DU
- Publication Type:Journal Article
- Keywords:
stem cells;
bone marrow;
mesenchymal stem cells;
celltransplantation;
transplantation,isogeneic;
autoimmunity
- From:
Chinese Journal of Tissue Engineering Research
2014;(1):106-111
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Mesenchymal stem cells are found to have the immunoregulatory activities and a potential application prospect in the treatment of autoimmune diseases.
OBJECTIVE:To explore the mechanism of transplanting mesenchymal stems cells on the treatment of multiple sclerosis.
METHODS:The mouse mesenchymal stems cells were prepared, and injected into the al ogenic and syngenic normal mice, to detect the frequency of CD4+CD25+Foxp3+T cells in the spleen, thymus, and lymph nodes by flow cytometry, and to detect the Foxp3, transforming growth factor-β1, and interleukin-10 mRNA in the spleen, thymus, and lymph nodes by reverse transcription-PCR.
RESULTS AND CONCLUSION:Transplantation of mesenchymal stem cells on normal mice led to a significant up-regulation of CD4+CD25+Foxp3+T cells, Foxp3, transforming growth factor-β1, and interleukin-10 mRNA in the spleen, thymus, and lymph nodes both in the al ogenic and syngenic transplant groups. Transplantation of mesenchymal stem cells may be an available method in the treatment of autoimmune diseases, and CD4+CD25+Foxp3+T cell, Foxp3, transforming growth factor-β1, and interleukin-10 may be involved in this process.
