Location and expression of cyclin A2 in neonatal mouse myocardium
10.3969/j.issn.2095-4344.2014.02.007
- VernacularTitle:乳鼠心肌组织中细胞周期素A2的定位及表达
- Author:
Aichao ZHAO
;
Yitong MA
;
Yongzhao YAO
;
Wen CAO
;
Haibin YU
;
Hui YU
;
Fen LIU
;
Bangdang CHEN
;
Xiang MA
- Publication Type:Journal Article
- Keywords:
cyclins;
cyclin A;
cyclin A2;
myocytes,cardiac;
proliferating cell nuclear antigen
- From:
Chinese Journal of Tissue Engineering Research
2014;(2):199-204
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Cyclin A2 is a key regulator of cellcycle. Location and expression of cyclin A2 in neonatal mouse myocardium is not clear.
OBJECTIVE:To observe the location and expression of cyclin A2 in neonatal mouse cardiomyocytes and its relationship with the exit of cardiomyocytes from cellcycle.
METHODS:Neonatal mice were kil ed to take myocardial tissues at 0, 3, 7, 14 and 28 days after birth. Western blot were used to detect the expression of cyclin A2, proliferating cellnucleus antigen and Phospho-histone H3. Immunohistochemitry detection was used to detect the location of cyclin A2 and expression of proliferation cellnucleus antigen at different time after birth.
RESULTS AND CONCLUSION:Western blot showed the decrease of cyclin A2 after birth til disappeared at day 4 (P=0.001). Cyclin A2 located mainly in the nucleus after birth and exported to the cytoplasm at day 14, and basical y disappeared at day 28. Proliferating cellnucleus antigen showed gradual y decreased tendency after birth. Mitosis specific marker, Phospho-histone H3, exhibited a gradual decrease after birth, which was consistent with cyclin A2 in expression intensity.
