Altered morphology in erythrocytes of autologous blood stored at different temperatures
10.3969/j.issn.2095-4344.2014.02.008
- VernacularTitle:自体血贮存在不同温度时红细胞形态的变化
- Author:
Guozhen LI
;
Li YUAN
;
Shanling LIU
;
Shiduan WANG
- Publication Type:Journal Article
- Keywords:
hemodilution;
blood preservation;
erythrocytes;
blood transfusion
- From:
Chinese Journal of Tissue Engineering Research
2014;(2):205-210
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Transfusion guidelines pointed out:whole blood should be stored at (4±2)℃. The bacterial growth or loss of function should occur if the blood leaves the suitable storage conditions. Recipients wil suffer from different degrees of blood transfusion reaction or invalid infusion.
OBJECTIVE:To observe morphology of erythrocytes of autologous blood stored at different temperatures using microscope.
METHODS:Blood was obtained from 40 cases of acute normovolemic hemodilution and stored in ACD citrate bags. Whole blood was respectively stored at 4 ℃ and 23 ℃. Blood smear was taken respectively in the blood storage immediately, 1, 2, 3, 4, 5 and 6 hours after col ecting autologous blood. Changes in morphology of erythrocytes were observed with a microscope. Deformity rate of erythrocytes was calculated. Six blood samples were randomly selected to test pH, K+, and free hemoglobin respectively in 6-hour common temperature group and ACD banked blood within the valid period. Six blood samples were randomly selected for the bacterial culture in each group of two groups at 6 hours.
RESULTS AND CONCLUSION:There were no significant differences in abnormality rates of erythrocytes between 4 ℃ and common temperature groups at each time point. The pH, K+, free hemoglobin at six hours in the common temperature group were better than those of ACD banked blood within the valid period and there was no bacterial growth in culture between the two groups. Therefore, it is feasible to transfuse autologous blood back to the patient within 6 hours of storage at room temperature.
