Preparation and identification of Env-pseudotyped subtype B HIV-1
10.7644/j.issn.1674-9960.2014.01.009
- VernacularTitle:HIV-1 B亚型假病毒的制备与鉴定
- Author:
Maopeng WANG
;
Chang LI
;
Shouwen DU
;
Yilong ZHU
;
Na ZHU
;
Dandan SUN
;
Ningyi JIN
- Publication Type:Journal Article
- Keywords:
Env-pseudotyped subtype B HIV-1;
infection;
identification
- From:Military Medical Sciences
2014;(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the Env-pseudotyped subtype B HIV-1 with enhanced green fluorescent protein ( EG-FP) gene,explore HIV-1 infection mechanisms and develop feasible methods of identification .Methods The Env-pseudo-typed viruses were packaged in HEK293T cells by cotransfection, and the reporter gene and P24 protein were detected by PCR, Western blot and ELISA .Reporter gene amplification , viral titration assay and a single round of infection assay were performed after the env-pseudotyped viruses infected HIV-1 permissive cell .Results and Conclusion A generation and identification method of the pseudotyped HIV-1 was established . The Env-pseudotyped subtype B HIV-1 has been prepared, which is able to infect SupT1 and TZM-bl cells through infection assay .
