Biological characterization of C57 mouse bone marrow mesenchymal stem cells using a whole bone marrow adherent culture technique
10.3969/j.issn.2095-4344.2014.01.008
- VernacularTitle:全骨髓法培养C57小鼠骨髓间充质干细胞的生物学特性
- Author:
Rongyao ZHANG
;
Xiaojuan BI
;
Yan MA
;
Xianlin DUAN
;
Ming JIANG
- Publication Type:Journal Article
- Keywords:
stem cells;
mesenchymal stem cells;
cellculture techniques;
induced pluripotent stem cells
- From:
Chinese Journal of Tissue Engineering Research
2014;(1):45-50
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Under mitomycin C treatment, feeder cells appear to have restricted proliferation, but they are stil able to secret different cytokines. Non-mesenchymal stem cells from the bone marrow and secreted factors in plasma maintain the micro-environment suitable for the growth of mesenchymal stem cells that can improve the yield of mesenchymal stem cells.
OBJECTIVE:To study the biological characteristics of C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique.
METHODS:Using the whole bone marrow adherent culture technique, purified and amplified C57 mouse bone marrow mesenchymal stem cells were harvested. cellproliferation kinetics, immune cellsurface markers, multiple differentiation potential and cellcycle were detected.
RESULTS AND CONCLUSION:Using the whole bone marrow culture, mouse bone marrow mesenchymal stem cells were harvested and capable of adhering to the plastic culture vessel. The obtained cells expressed CD45, CD105 and Sca-1, but were negative for CD34, CD33 and C-kit. The doubling time was (57.11±1.5) hours. The cells could be induced to differentiate into osteoblasts, adipocytes and chondrocytes. The cellcycle analysis showed that 64%of cells were in G 0-G 1 phase. These indicates that C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique have biological characteristics of mesenchymal stem cells.
