The expression and clinical signification of tM2-PK in colorectal cancer
- VernacularTitle:tM2-PK蛋白在结直肠癌中的表达及临床意义
- Author:
Xin YU
;
Yi LIU
- Publication Type:Journal Article
- Keywords:
colorectal carcinoma;
tumor M2 pyruvate kinase;
IHC;
ELISA
- From:
Acta Universitatis Medicinalis Anhui
2014;(2):236-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical signification of tumor M2 pyruvate kinase in pa-tients with CRC, and to try to find a reliable and atraumatic method to predict the progress and pronosis in the CRC. Methods ELISA method was used to determine the serum levels of tM2-PK in 68 patients with CRC,40 pa-tients with colorectal polyps and 40 cases of normal subjects; immunohistochemistry ( IHC ) methods were respec-tively used to detect the expression of tM2-PK in tissues. Results ① The level of serum tM2-PK in patients with CRC was significantly higher than the group of patients with polyps and the normal subjects ( P<0.05 ) . The serum tM2-PK levels was closely correlated to tumor size and Duke stage.②The positive rate of tissue tM2-PK was signif-icantly higher in CRC than in colorectal polyps and normal subjects ( P<0.05 ) . Tissue tM2-PK levels was closely correlated to tumor size,Duke stage and differentiation grade. ③Serum tM2-PK levels was correlated to tissue tM2-PK levels (r=0.357,P=0.003). Conclusion Combined detection of expression of tM2-PK can provide the basis theory for the diagnosis and prognosis evaluation of CRC.
