The targeting expression of IL-2 gene driven by alpha fetoprotein promoter and hepatitis B virus envelope in hepatic carcinoma cell
10.3760/cma.j.issn.1006-9801.2014.01.006
- VernacularTitle:乙肝病毒衣壳运载体甲胎蛋白启动子驱动的白细胞介素-2在肝癌细胞中的靶向表达
- Author:
He WANG
;
Zhihua DENG
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
HBVE;
Alpha-fetoproteins;
Promoter regions (genetics);
Interleukin-2;
Targeting
- From:
Cancer Research and Clinic
2014;26(1):20-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe if IL-2 gene can express in hepatic carcinoma cells by double targeting of hepatitis B virus envelope (HBVE) and alpha fetoprotein (AFP) promoter.Methods HepG2 cells,L02 cells,and HepG2.2.15 cells were cultured in vitro.HBVE was obtained by PEG8000 concentration assay,and the acquired HBVE was used to pack recombintional gene.Human AFP promoter-IL-2 recombinational gene was obtained by PCR.Then HepG2 cells and L02 cells were transfected by transient transfection and the expression of IL-2 was detected by RT-PCR and Western blot.Results IL-2 was detected in HepG2 cells by RT-PCR and Western blot but not in L02 cells.Conclusion By using HBVE as a gene transporter,human AFP promoter-IL-2 recombinational gene can express in hepatic carcinoma cells,thereby it can increase the safety of exogenous gene transfection of hepatic carcinoma cells.
