Intra-pulmonary arterial antitumor necrosis factor-α antibody effect on ultra-structure of lung following cardiopulmonary bypass
10.3760/cma.j.issn.1001-4497.2014.01.010
- VernacularTitle:肺灌注抗肿瘤坏死因子抗体对体外循环肺超微结构的影响
- Author:
Mingxin GAO
;
Haitao LI
;
Fan ZHANG
;
Chen BAI
;
Yang YU
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary bypass;
Lung injury;
Apoptosis;
Ultra-structure;
Anti-tumor necrosis factor antibody
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2014;30(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of intra-pulmonary arterial anti-tumor necrosis factor-α antibody(TNF-α Ab) on ultra-structure of lung after cardiopulmonary bypass (CPB).Methods Forty New Zealand rabbits were selected and randomly divided into four groups:group Ⅰ-Ⅲ underwent CPB; group Ⅳ only received open chest operation.In the group Ⅱ,rabbit TNF-α Ab(27 ng/kg) was dropped into the pulmonary artery when the aorta was clamped and CPB continued 30 minute.Pulmonary arterial perfusion was given to the group I instead.Blood TNF-α and neutrophils count from right and left atrium and oxygenation index in the four groups were determined perioperatively.Lung water content,TNF-αmessenger RNA,Bcl-2 protein,Bax protein,Bcl-2/Bax,apoptosis index and pathomorphological change were measured in the lung tissues.Results TNF-α Ab can restrain leukocyte accumulation and reduce releasing of TNF-α in the lung and improve oxygenation index.Moreover,TNF-α Ab can reduce the expression of Bax on alveolar epithelial cells and alleviate the decreasing of the expression of Bcl-2.It can also reduce the occurrence of apoptosis and attenuate pathomorphological changes in the lung tissue.Conclusion Intra-pulmonary arterial antitumor necrosis factor-α antibody markedly lessens the injury of inflammatory reaction and ultra-structure of lung after CPB via Bcl-2/Bax pathway.
