The effect of PTEN expression downregulation on biological characteristics of breast cancer cell line

VernacularTitle:PTEN基因表达下调对乳腺癌细胞生物学性状的影响
Author: Xiaoxin JI ; Chengyu LUO ; Deming YU ; Xuan WU
Publication Type:Journal Article
Keywords: Breast neoplasms; Neoplasm metastasis; PTEN gene
From: Chinese Journal of General Surgery 2014;29(1):49-53
CountryChina
Language:Chinese
Abstract: Objective To investigate expression of the phosphatase and tensin homolog (PTEN) gene in breast cancer cell line and its effect on biologic characteristics.Methods The normal PTEN expression cell line MDA-MB-231 (M231) was used in this study.PTEN-shRNA plasmid was transected into M231 breast cancer cells to knock down the expression of PTEN.The changes of PTEN expression,proliferation,invasion and metastasis of PTEN knocked down cell were tested by RT-PCR,Western blot,CCK-8,scratch and Transwell.Results PTEN-shRNA was successfully transected into M231 cells.PTEN mRNA and protein expression was efficiently inhibited in M231-3001 cell lines than that in control group M231-scr(P ＜ 0.01),M231-3001 cell lines showed a greater capability of colony formation,migration and invasion than that in control group M231-scr (all P ＜ 0.05).Conclusions PTEN,as a suppression gene,its low expression can promote the proliferation,migration and invasion of breast cancer cells.