The regulative effect of costimulatory molecules B7-H1 on immunosuppressive function of myeloid-derived suppressor cells
10.3760/cma.j.issn.0254-9026.2014.02.024
- VernacularTitle:共刺激分子B7-H1对小鼠髓源性抑制细胞免疫抑制功能的调节作用
- Author:
Hongmei LIU
;
Ling WANG
;
Guangbo ZHANG
;
Siwen CHEN
;
Xudong PAN
;
Yanqing MAO
- Publication Type:Journal Article
- Keywords:
Suppressor factors,immunolagic;
Antigens,CD;
Immune tolerance
- From:
Chinese Journal of Geriatrics
2014;33(2):197-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of negative costimulatory molecule B7-H1 of myeloid derived suppressor cells(MDSCs) and its roles in age-related immunosuppressive functions.Methods 36 C57BL/6 mice were divided into 2 groups:the elderly group(12-month-old,n=18) and the young group(4-8-week-old,n=18).The expressive level of negative costimulatory molecules of MDSCs in the two groups was measured by flow cytometry(FCM).Besides,MDSCs were sorted by using MDSC isolation kit and T cells were obtained by grinding lymph nodes.To explore the influence of B7-H1 on the MDSCs' immunosuppressive functions,T cell proliferation and intervention assay was conducted using the five following groups:T cells group,T cells + CFSE group [T cells with carboxyfluorescein diacetate succinimidyl ester(CFSE)],T cells +-CFSE cocultured with MDSCs from the young group,T cells + CFSE cocultured with MDSCs from the elderly group,and T cells + CFSE cocultured with MDSCs from elderly group and anti-B7-H1 blocking antibody.The effect of B7-H1 on T cell proliferation in MDSCs were observed.Results The expression level of B7-H1 of the MDSCs was significantly increased in the elderly group as compared with the young group(t=3.27,P＜0.05).T cell proliferation assay revealed that T cells were remarkably suppressed by MDSCs in elderly group as compared to the youth group(t=5.42,P＜0.05).The suppression of T cells by MDSCs was dramatically reversed by anti-B7-H1 blocking antibody in elderly group(t=8.28,P＜0.05).Conclusions B7-H1,a molecule expressed on the surface of MDSCs,plays a key role in the age-related immunosuppressive function of MDSCs.
