Regulatory B Cells Are Inversely Associated with Disease Activity in Rheumatoid Arthritis.
10.3349/ymj.2014.55.5.1354
- Author:
Jinhyun KIM
1
;
Hyun Ji LEE
;
In Seol YOO
;
Seong Wook KANG
;
Jae Ho LEE
Author Information
1. Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Regulatory B lymphocytes;
rheumatoid arthritis;
interleukin-10
- MeSH:
Adult;
Aged;
Antigens, CD19/metabolism;
Arthritis, Rheumatoid/blood/*immunology/pathology;
B-Lymphocytes, Regulatory/metabolism/*physiology;
Biological Markers/blood;
Female;
Humans;
Interleukin-10/metabolism;
Male;
Middle Aged;
Severity of Illness Index
- From:Yonsei Medical Journal
2014;55(5):1354-1358
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The function of regulatory B lymphocytes is known to be abnormal in inflammatory diseases. However, a recent study indicates that IL-10+ B cells seem to be expanded in rheumatoid arthritis (RA). Therefore, the state of IL-10+ B cells in the peripheral blood from RA patients and healthy controls were investigated. MATERIALS AND METHODS: CD19+ cells in peripheral blood mononuclear cells were purified from blood samples of RA patients and age and gender-matched healthy controls, and stimulated with CD40 ligand and CpG for 48 hours. Then, intracellular IL-10 in CD19+ cells was analyzed using flow cytometry. RESULTS: There was no significant difference in the proportion of IL-10+ B cells between 10 RA patients and 10 healthy controls (RA, 0.300+/-0.07 vs. healthy control 0.459+/-0.07, p=0.114). The proportion of induced IL-10+ B cells to total B cells in RA patients was significantly higher than those in controls (RA, 4.44+/-3.44% vs. healthy control 2.44+/-1.64%, p=0.033). However, the proportion of IL-10+ B cells to total B cells correlated negatively with disease activity in RA patients (r=-0.398, p=0.040). Erythrocyte sedimentation rate or C-reactive protein or medication was not associated with the proportion of IL-10+ B cells. CONCLUSION: The proportion of induced IL-10+ B cell increased in RA patients compared to healthy control, however, negatively correlated with disease activity in RA.
