Study on PLGA nanoparticle loaded with gold nanorod and liquid perflurocarbon for photoacoustic imaging
10.3760/cma.j.issn.1004-4477.2014.02.016
- VernacularTitle:包裹金纳米棒的PLGA液态氟碳纳米粒在光声成像中的实验研究
- Author:
Lei SU
;
Haitao RAN
;
Zhigang WANG
;
Yuping GONG
;
Chengbo LIU
;
Bin ZHANG
- Publication Type:Journal Article
- Keywords:
Photoacoustic imaging;
Gold nanorod;
Vaporization
- From:
Chinese Journal of Ultrasonography
2014;23(2):154-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize a photoacoustic contrast agent loaded with gold nanorod and liquid perflurocarbon which was used to observe its photoacoustic imaging in vitro,and then investigate the variation of the photoacoustic signal during and after the vaporization.Methods The PLGA nanoparticle (GNP)loaded with gold nanorod(GNR) and liquid perflurocarbon was synthesized via double emulsion technology.The experiments were divided into three groups:1)GNP group:PLGA nanoparticle loaded with gold nanorod and liquid perflurocarbon; 2) GNR group:gold nanorod ; 3) control group:PLGA nanoparticle unloaded with gold nanorod.A pulsed laser system was used to trigger the three samples.The photoacoustic image and time-signal intensity kinetics were acquired by the photoacoustic microscope.The optical microscope was used to observe the image after the vaporization.Results The gold nanoparticle was successfully prepared,the average size of the gold nanoparticle was 504.9 nm.after triggered by the pulsed laser system,the vaporized image of the GNP group could be observed by the optical microscope.The photoacoustic signal was detected in both GNP and GNR groups,and the signal intensity increased along with the concentration of the GNR.However,the photoacoustic signal of PLGA group was not detected.The photoacoustic signal kinetics of GNP group showed a descending trend from high signal amplitude to steadystate.The GNR group photoacoustic signal kinetics only showed a steady-state signal amplitude.Conclusions The PLGA nanoparticle loaded with gold nanorod and liquid perflurocarbon emerged a phase transient which produces a strong photoacoustic signal after triggered by pulsed laser system.This kind of phenomenon leads the nanoparticle to be a better photoacoustic contrast agent and lays a foundation for in vivo research and photothermal therapy in vivo.
