The relationship between inhibition of hepatitis B virus replication induced by tumor-necrosis factor α and the up-regulated proprotein convertases
10.3760/cma.j.issn.1000-6680.2014.02.005
- VernacularTitle:肿瘤坏死因子-α抑制乙型肝炎病毒复制的作用与其上调原蛋白转化酶的关系
- Author:
Yan CHEN
;
Hong SHI
;
Lin GU
;
Xiaomou PENG
- Publication Type:Journal Article
- Keywords:
Proprotein convertases;
RNA,messenger;
Tumor necrosis factor-alpha;
Hepatitis B virus;
Virus replication
- From:
Chinese Journal of Infectious Diseases
2014;32(2):85-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of tumor-necrosis factor α (TNF-α) on the expressions of proprotein convertases (PC) and its relationship with the inhibition of hepatitis B virus (HBV) replication.Methods HepG2.2.15 cells cultured routinely were exposed to 20 μg/L recombinant TNF-α and/or 20 μmol/L PC inhibitor (DEC) for 18 h.Then Followed cells werecollected and cell total RNA and HBV DNA were extracted.PC mRNA and core-associated HBV DNA were measured using real-time polymerase chain reaction (PCR) techniques.Measurement data was compared using t-test.Results When PC mRNA expressions in the blank group was as to 1,the expressions of PC1/3、PC2、furin、PC4 、PC5/6 、PACE4 and PC7/8 mRNA in HepG2.2.15 cells treated with 20μg/L TNF-α treatment for 18 h were all up-regulated,which were 3.3±0.7、79.3±3.3、77.5±1.3、19.2±3.1、1.3±0.1、1.4± 0.2、274.8± 7.1,respectively (all P＜0.05).Treatment of 20 μg/L recombinant TNF-α for 18 h significantly reduced core-associated HBV DNA compared with blank gourp (0.21∶1,t =8.79,P =0.002),while 20 μmol/L DEC significantly up-regulated core-associated HBV DNA (3.84∶ 1,t=7.67,P=0.004).Moreover,core-associated HBV DNA in group of DEC and TNF-α treatment was significantly higher than group of TNF-α treatment (0.31∶0.21,t=10.49,P=0.007).Conclusion Up-regulated PC mRNA expression induced by TNF-α is significantly associated with the inhibition of HBV replication.
