Effect of chronic stress on expression of uncoupling protein 4 and Bcl-2 protein of rat hippocampal mitochondria
10.3760/cma.j.issn.1674-6554.2014.02.002
- VernacularTitle:慢性应激对大鼠海马线粒体解偶联蛋白4及Bcl-2蛋白表达的影响
- Author:
Geng CHANG
;
Jing XU
;
Wendi ZHANG
;
Zhen MU
;
Yi CHANG
- Publication Type:Journal Article
- Keywords:
Depression;
Chronic unpredicted mild stress;
Mitochondria;
Hippocampus;
Uncoupling Protein 4
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;23(2):101-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of chronic stress on the expression of uncoupling protein 4 (UCP4) and Bcl-2 protein in hippocampal neurons of rat depression model.Methods Rat depression models were established by chronic unpredicted mild stress.All rats were randomly assigned to 2 groups:control group and model group.Flow cytometry was applied to detect the apoptosis rate and mitochondrial membrane potential.The level of LDH was measured by enzymes labelling instrument.The number of neurons was measured by immunohistochemistry.The expression of UCP4 and Bcl-2 protein was measured by Western blotting.Results After chronic stress,the apoptosis rate((4.35±0.19) ％)and LDH activity ((445.50±91.70) U/mg) in hippocampal tissue in the model group was significantly higher than the control group((0.34±0.06) ％,(167.20±63.40)U/mg).Compared to control group,the number of hippocampal neurons ((72.50±4.25) vs (45.30±2.54)) and the mitochon drial membrane potential decreased in the model group.The expressions of UCP4 and Bcl-2 protein in hippocampal tissue were significantly lower than the control group.Conclusion Chronic unpredicted mild stress can lead to apoptosis in rat hippocampal neurons,which is related with decline of mitochondrial membrane potential and low expression of UCP4 and Bcl-2 protein.
