Effects of insulin-like growth factor-1 on the nerve function and expression of basic fibroblast growth factor after cerebral ischemic reperfusion in rats
10.3760/cma.j.issn.1674-6554.2014.02.005
- VernacularTitle:胰岛素样生长因子-1对局灶性脑缺血大鼠神经功能评分及碱性成纤维生长因子表达的影响
- Author:
Li GUO
;
Yanfeng GUO
;
Zongmao ZHAO
;
Shucheng MA
;
Guozhu SUN
;
Xiaopeng LIU
- Publication Type:Journal Article
- Keywords:
Ischemia;
Insulin-like growth factor-1;
Basic fibroblast growth factor
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;23(2):110-113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe dynamically the influence of Insulin-like growth factor-1 (IGF-1) on the nerve function and expression of bFGF protein and Basic fibroblast growth factor(bFGF) mRNA after cerebral ischemic reperfusion in rats.Methods Seventy-two SD rats were randomly divided into sham-operated group,cerebral ischemia group,and IGF-1 treated group.The rat model of middle cerebral artery occlusion (MCAO) and reperfusion was performed.The evaluation of etiology was performed with mNSS at 12 h,24 h,3 d,7 d after ischemia-reperfusion,expression of bFGF protein was determined with immunohistochemical technique and expression of bFGF mRNA was determined with RT-PCR.Results The ratings of mNSS in IGF-1 treated group((8.67± 1.21),(7.50± 1.52),(4.33± 1.03),(3.67± 1.37)) were lower than those in ischemia group((11.0±1.26),(9.83±1.33),(7.83±1.17),(7.17±1.72) at 12 h,24 h,3 d or7 d after reperfusion(P＜0.05).For the IGF-1 treated group,the expression level of bFGF protein was higher than that of the cerebral ischemia group (P＜0.05),especially at 12 h after reperfusion (P＜0.01).The expression level of bFGF mRNA in the IGF-1 treated group was higher than that of the cerebral ischemia group (P＜ 0.05),especially at 24h after reperfusion (P＜ 0.01).Conclusion IGF-1 treatment has a protective effects on cerebral ischemia injury,which may contribute to its action on regulating expression of bFGF protein and bFGF mRNA.
