The effect of transmembrane tumor necrosis factor-alpha on human cervical carcinoma HeLa cells
10.3760/cma.j.issn.1673-4904.2014.09.002
- VernacularTitle:跨膜型肿瘤坏死因子-α对宫颈癌细胞毒性效应的研究
- Author:
Xiaojun CAI
;
Lin ZHANG
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-alpha;
Uterine cervical neoplasms;
HeLa cells
- From:
Chinese Journal of Postgraduates of Medicine
2014;37(9):4-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of transmembrane tumor necrosis factor-alpha (TM-TNF-αt) on human cervical carcinoma HeLa cells,and explore the correlations with silencer of death domain (SODD).Methods The expression of proliferating cell nuclear antigen (PCNA) was detected by immunocytochemistry.The expression of SODD in human cervical carcinoma HeLa cells undisposed and after disposed with TM-TNF-α was detected by reverse transcription-polymerase chain reaction,and the influence of TM-TNF-α on human cervical carcinoma HeLa cells was analyzed.Results The positive rate of PCNA in undisposed human cervical carcinoma HeLa cells was 80.3％ (155/193),in human cervical carcinoma HeLa cells disposed with TM-TNF-α was 46.7％ (85/182),there was statistical difference (P ＜ 0.05).The cycle index of polymerase chain reaction in human cervical carcinoma HeLa cells was 28 times,the amplification product was disposed by 2％ agarose gel electrophoresis,the gray scale disposed by TM-TNF-α and undisposed human cervical carcinoma HeLa cells were 1.377 ± 0.170 and 0.815 ± 0.040,there was statistical difference (P ＜ 0.05).Conclusion TM-TNF-α has obvious cytotoxic effect on human cervical carcinoma HeLa cells in vitro which may due to its up-regulating the expression of SODD.
