Pioglitazone inhibits atrial ionic channel remodeling in diabetic rabbits hearts
10.3760/cma.j.issn.1671-0282.2014.02.015
- VernacularTitle:吡格列酮抑制糖尿病兔心房肌细胞离子通道重构
- Author:
Changle LIU
;
Guangping LI
;
Huaying FU
;
Jian LI
;
Lijun CHENG
;
Wansong YANG
;
Tong LIU
- Publication Type:Journal Article
- Keywords:
Atrial fibrillation;
Diabetes mellitus;
Pioglitazone;
Patch-clamp technique;
Action potential duration;
L-type calcium channel current;
Rapid sodium channel current;
Lonic channel;
Remodeling
- From:
Chinese Journal of Emergency Medicine
2014;23(2):181-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pioglitazone on atrial ionic channel remodeling in alloxan-induced diabetic rabbit models.Methods A total of 32 rabbits were randomly (random number) divided into control (CN) group,diabetes mellitus (DM) group,diabetes mellitus + pioglitazone 4 mg/ (d · kg) (DPG) group and diabetes mellitus + double pioglitazone 8 mg/ (d · kg) (DPI) group.The diabetic state was examined by quantitative determination of blood glucose levels of ≥ 14 mmol/L.Langendorff-perfused rabbit hearts were used to isolate single atrial myocyte,and whole-cell patch-clamp technique was used to record action potential duration (APD) and atrial ionic channel currents (ICa,L and INa).Variables with normal distribution were compared with One-way ANOVA and LSD-t test.Results Compared with controls,APD90 and APDS0 of left atrial myocytes were significantly prolonged in DM group (P ＜0.05 vs.CN),and there was no significant difference in APD90 frequency adaptation between them (P ＞0.05 vs.CN).The densities of INa were reduced and the densities of ICa,L were increased in DM group (P ＜ 0.01 vs.CN).The above variables were markedly attenuated in DPG and DPI group.Conclusions Pioglitazone may inhibits atrial ionic channel remodeling in diabetic rabbit models.
