Effects of hydrogen sulfide on apoptosis of cardiomyocytes after cardiopulmonary resuscitation in a rat model
10.3760/cma.j.issn.1671-0282.2014.01.006
- VernacularTitle:硫化氢对大鼠心肺复苏后心肌细胞凋亡的影响
- Author:
Xuemeng XIE
;
Hao PAN
;
Beibei LIU
;
Di CHEN
;
Jincheng ZHANG
;
Guangtian YANG
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary resuscitation;
Myocardial dysfunction;
Hydrogen sulfide;
Apoptosis
- From:
Chinese Journal of Emergency Medicine
2014;23(1):19-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hydrogen sulfide (H2S) on apoptosis of cardiomyocytes after cardiopulmonary resuscitation (CPR) in rat models.Methods Forty-five male SD rats were randomly into sham group (n =15),CPR group (n =15) and NaHS group (n =15).Rats of CPR group and NaHS group were operated to induce cardiac arrest by transcutaneous electrical stimulation to epicardium.In NaHS group,NaHS (5 mg/kg) was administrated via the femoral venous line 1 min before CPR.Hemodynamic variables were monitored and obtained continuously.Survival rats were sacrificed at 24 h after restoration of spontaneous circulation and the hearts were removed for analysis by RT-PCR and TUNEL assays.Blood samples were collected and plasma content of cTnT was detected.Results Compared with the CPR group,animals treated with NaHS had improved left ventricular function (P ＜0.01),lower plasma cTnT levels (P ＜0.05) and decreased apoptosis index (P ＜ 0.01) 24 h after ROSC.The expressions of Caspase-3 mRNA,Bax mRNA and Bcl-2 mRNA in CPR group and NaHS group were higher compared with the control group (P ＜0.01).The NaHS group had lower expressions of Caspase-3 mRNA and Bax mRNA (P ＜0.01),but higher expression of Bcl-2 mRNA (P ＜0.05) compared with the CPR group.Conclusions Exogenous (H2S) regulated the expressions of Caspase-3,Bax and Bcl-2 mRNA,thereby preventing apoptosis of cardiomyocytes,inhibiting cTnT release and improving left ventricular function 24 h after CPR.
