Isolation, expansion and adipogenic differentiation of human keloid-derived precursor cells
10.3760/cma.j.issn.1671-0290.2013.05.015
- VernacularTitle:人体瘢痕疙瘩前体细胞的分离培养及向脂肪细胞的定向诱导分化
- Author:
Xunxun LIN
;
Jie LIU
;
Fugui LI
;
Ridong WU
;
Shi TANG
;
Qing TANG
- Publication Type:Journal Article
- Keywords:
Keloid;
Adipogenic differentiation;
Mesenchymal stem cell (MSC)
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2013;19(5):368-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the isolation,amplification methods and adipogenic differentiation under specific culture medium of human keloid-derived precursor cell (KPC) in vitro,in order to study their possibility of being new seed cells of tissue engineering fat.Methods KPCs were isolated from human keloid tissue of 4 different patients in our hospital and were cultured in the modified L-DMEM culture medium.Their cloning efficiency and growth curve were tested.The subcultured cells were tested of the mesenchymal stem cell (MSC)-related gene expression by flow cytometry.In addition,they were cultured in H-DMEM medium (containing 1 μmol/L dexamethasone,0.5 mmol/L 3-isobutyl-1-methyl-xanthine 10 mg/L of bovine insulin,100 mmol/L indomethacin,and 10 ％ FBS)and were later observed in oil red O staining under phase contrast microscope to determine whether lipid droplets generation was formed,using skin-derived precursors (SKP) as control.Results More than 95 ％ KPC expressed many antigens of MSC,such as CD29,CD44,CD90 and CD105 while few of them expressed CD34,CD45(1.0 ％-2.5 ％).And the cells increased in size gradually after inducted the same time,changing from spindle into round or polygonal in shape.The lipid droplets were seen in 72 hours and expressed a positive rate of 78.6 ％ in Day 19 in oil red O staining while the same rate was 54.6 ％ in SKP.Conclusions Human keloid-derived precursor cells can express a variety of MSC-related surface markers without expressing hematopoietic stem cell (HSC) related markers.Furthermore,they can be differentiated into fat cells under certain conditions,which may make them as a new source of seed cells for tissue engineering fat.
