Effects of D-cresol on the proliferation and cell cycle of human umbilical vein endothelial cells

VernacularTitle:对甲酚对人脐静脉内皮细胞增殖和细胞周期的影响
Author: Li LI ; Lixia GUANG ; Daihong WANG ; Xiang DU ; Fahuan YUAN
Publication Type:Journal Article
Keywords: Endothelial cells; Cell proliferation; Cell cycle; P-cresol
From: Chinese Journal of Nephrology 2013;29(9):676-680
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of p-cresol on human umbilical vein endothelial cells.Methods The effects of p-cresol on endothelial cell growth,cell cycle,cell morphological change and p21 protein were detected by the CCK-8 assay,flow cytometry assay,inverted microscope and Western blotting.Results P-cresol could inhibit the growth of human umbilical vein endothelial cells in dose-and time-dependent manners (all P ＜ 0.05).The human umbilical vein endothelial cells treated with p-cresol became elongated processes,cloudy cytoplasm,and irregular shapes.The p-cresol stopped human umbilical vein endothelial cells at cell cycle G1 and had no effect on cell apoptosis.The p-cresol could increase protein expression of p21 in a dose dependent manner (P ＜ 0.05).Conclusion P-cresol can increase protein expression of p21,induce cell cycle arrest at G1 stage and inhibit the proliferation of human umbilical vein endothelial cells.