Effects of berberine on autophagy in C3H10T1/2 adipocytes

VernacularTitle:小檗碱对C3H10T1/2脂肪细胞自噬的影响
Author: Xiaoyan ZHANG ; Yujie DENG ; Jian YANG ; Pengfei Lü ; Ying YANG
Publication Type:Journal Article
Keywords: Berberine; Autophagy; Adipocytes
From: Chinese Journal of Endocrinology and Metabolism 2013;29(10):890-894
CountryChina
Language:Chinese
Abstract: Objective To assess the effects ofberberine on autophagy in C3H10T1/2 adipocytes.Methods C3H10T1/2 cells,a pluripotent stem-cell line of mesodermal origin,were induced to differentiate into mature adipocytes,and then were treated with berberine,The expression of autophagy marker protein LC3 Ⅱ/Ⅰ and autophagy substrate P62 was determined by Western blot ; after treating C3 H 10T1/2 cells with berberine and lysosomal inhibitor,chloroquine,autophagy flux was assessed by Western blot.Autophagosome was observed by transmission electron microscopy after berberine treatment.Results (1) After berberine treatment,the expression of LC3 Ⅱ / Ⅰin C3H10T1/2 adipocytes was reduced and P62 was increased in a time-and dose-dependent manner(P＜0.05 or P＜0.01) ; (2) Following treatment with chloroquine and berberine,the protein level of LC3 Ⅱ/Ⅰ was decreased (P＜0.05); (3) The number of autophagosome was decreased apparently after berberine treatment.Conclusions Berberine inhibits autophagy in C3H10T1/2 adipocytes.