Study on differentially expressed serum proteins in Uyghur patients with primary hyperuricemia
10.3760/cma.j.issn.1000-6699.2013.09.015
- VernacularTitle:维吾尔族原发性高尿酸血症血清差异蛋白质研究
- Author:
Fei YU
;
Zhaoyun CHEN
;
Zhaoxia ZHANG
;
Jing TANG
;
Wei LI
;
Hua YAO
- Publication Type:Journal Article
- Keywords:
Hyperuricemia;
Uyghur;
Serum;
Proteomics
- From:
Chinese Journal of Endocrinology and Metabolism
2013;29(9):792-794
- CountryChina
- Language:Chinese
-
Abstract:
2-Dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS) were used to screen differentially expressed serum proteins in Uyghur patients with hyperuricemia and control subjects.Western blot was used to validate differential proteins.Gel-image analysis revealed that there were 11 differential protein spots.A total of 4 differential protein spots were successfully identified by MALDI-TOF-MS.Complement 3,complement 4,haptoglobin,and apolipoprotein Al levels were up-regulated in patients as compared with controls.The result of complement 3 expression was validated by Western blot,which is accordant with that of proteomics.
