Expression of interleukin-17 in diabetic macroangiopathy and the mechanism of intervention with resveratrol
10.3760/cma.j.issn.1000-6699.2013.08.016
- VernacularTitle:白细胞介素17在糖尿病大血管中的表达及白藜芦醇的干预机制
- Author:
Xudan LOU
;
Haidong WANG
;
Shijin XIA
;
Qin GU
- Publication Type:Journal Article
- Keywords:
Interleukin-17;
Diabetic macroangiopathy;
Resveratrol;
DNA methylation
- From:
Chinese Journal of Endocrinology and Metabolism
2013;29(8):700-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of interleukin-17 (IL-17) in diabetic rat aorta and the effect of intervention with resveratrol,meanwhile,to explore the potential mechanisms of IL-17 induced diabetic vascular diseases and the protective role played by resveratrol in the epigenetic field.Methods The experiment was carried out in 4 groups:normal control group(NC),normal interventional group(NB),diabetic group(DM),and diabetic interventional group(DB),NB and DB groups were intervened with resveratrol.Immunohistochemistry was used to observe the histological localization of IL-17 and to measure the thickness of rat abdominal aorta.Western blotting,real-time PCR,and methylation-specific PCR were used respectively to compare the expression of IL-17 protein and mRNA,as well as DNA methylation in 4 groups.Results IL-17 mainly expressed in arterial intima of diabetic rats,the abdominal aorta in DM group was obviously thicker than that in NC and DB groups(P＜0.05).IL-17 protein and mRNA expressions in DM group were significantly higher than NC group(P＜0.05),and were reduced in NB and DB groups compared with NC and DM groups respectively.While DNA methylation levels of IL-17 in DM group were significantly lower than NC group(P＜0.01),however,the levels in NB and DB groups were elevated accordingly as compared with corresponding groups.Conclusions The increased levels of IL-17 in aorta of diabetic rats suggests that IL-17 is involved in the process of inflammatory responses to diabetic macrovascular diseases,while resveratrol could inhibit the expression,it may play a role in protecting aorta,and the regulation of IL-17 gene promoter DNA methylation levels may be the potential mechanism underlying these two phenomena.
