Role of beta3 subunit of voltage-gated sodium channels in neuropathic pain in mice

VernacularTitle:电压门控钠离子通道β3亚基在小鼠神经病理性痛中的作用
Author: Xuan ZHAO ; Hong TAN ; Lu ZHANG ; Zhiyu CHEN ; Yingwei WANG ; Weifeng YU
Publication Type:Journal Article
Keywords: Sodium channels; Neuralgia
From: Chinese Journal of Anesthesiology 2013;33(7):864-865
CountryChina
Language:Chinese
Abstract: Objective To evaluate the role of the beta3 subunit of the voltage-gated sodium channel (Scn3b) in neuropathic pain in mice.Methods The target gene Scn3b was embedded in the vector pBROAD-mcs and pBROAD3-mcs-Scn3b plasmid was then obtained.The primary mice were bred.The primary mice mated with C57/B6 mice and the transgenic mice were then generated.DNA,RT-PCR and Western blot experiments were performed to confirm the mice in which Scn3b was over-expressed.The mice with Scn3b over-expression multiplied rapidly to carry out the follow-up experiment.Ten transgenic mice (Scn3b group) and 10 control mice in the same litter (Con group) of both sexes,aged 2 months,weighing 25-30 g,were randomly chosen to establish the model of neuropathic pain.The mechanical pain threshold was measured before operation and on 3,5,7 and 14 days after operation.Results There was no significant difference in the mechanical pain threshold at each time point between the two groups (P ＞ 0.05).Conclusion Scn3b is not involved in the development and maintenance of neuropathic pain in mice.