Time Dependent Bladder Apoptosis Induced by Acute Bladder Outlet Obstruction and Subsequent Emptying is Associated with Decreased MnSOD Expression and Bcl-2/Bax Ratio.
10.3346/jkms.2010.25.11.1652
- Author:
Wen Ji LI
1
;
Mi Kyung SHIN
;
Seung June OH
Author Information
1. Department of Urology, Seoul National University Hospital, and Neuroscience Research Institute, Medical Research Center, Seoul National University, Seoul, Korea. sjo@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Urinary Bladder Neck Obstruction;
Apoptosis;
Oxidative Stress;
Poly (ADP-ribose) Polymerases
- MeSH:
Animals;
*Apoptosis;
Caspase 3/metabolism;
Male;
Oxidative Stress;
Poly(ADP-ribose) Polymerases/metabolism;
Proto-Oncogene Proteins c-bcl-2/*metabolism;
Rats;
Rats, Sprague-Dawley;
Superoxide Dismutase/*metabolism;
Time Factors;
Urinary Bladder/enzymology/metabolism;
Urinary Bladder Neck Obstruction/enzymology/*metabolism;
bcl-2-Associated X Protein/*metabolism
- From:Journal of Korean Medical Science
2010;25(11):1652-1656
- CountryRepublic of Korea
- Language:English
-
Abstract:
Ischemia/reperfusion (I/R) injury-induced oxidative stress plays an important role in the functional impairment of the bladder following acute bladder outlet obstruction (BOO) via induction of apoptosis. The purpose of this study was to investigate the time course of the bladder apoptosis, and apoptosis related molecular changes in the early stage of acute BOO. Twelve-week-old male Sprague Dawley rats were divided into control, acute BOO only (I), and acute BOO plus subsequent emptying (I/R) for 30, 60, 120 min, 3 days and 2 weeks. We examined the extent of bladder apoptosis, expression of Mn-superoxide dismutase (Mn-SOD), Bcl-2, Bax, caspase 3 and poly (ADP-ribose) (PAR) in the bladder. Bladder apoptosis was significantly increased in the I/R group at 30, 60, and 120 min following bladder emptying. BOO plus subsequent emptying for 30, 60, 120 min showed significant decrease in MnSOD and Bcl-2 expression, and significant increase in caspase 3, Bax expression, and amounts of PAR. These results indicate that bladder apoptosis, induced by acute BOO and subsequent emptying, is associated with decreased MnSOD expression, increased PARP activity and imbalance in apoptosis pathways.
