Expression of nucleolar spindle-associated protein 1 in hepatocellular carcinoma and its clinical significance
10.3760/cma.j.issn.1007-8118.2013.10.008
- VernacularTitle:核仁纺锤体相关蛋白1在肝细胞癌中的表达及临床意义
- Author:
Meng ZHANG
;
Dinghua YANG
;
Xiao LIU
;
Yan LIU
;
Jiankun LIANG
;
Huanxin LIU
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Nucleolar spindle-associated protein 1
- From:
Chinese Journal of Hepatobiliary Surgery
2013;19(10):746-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of nucleolar spindle-associated protein 1(NuSAP1) in hepatocellular carcinoma (HCC) and in non-cancerous tissues,and to study the correlation between NuSAP1 and early recurrence and prognosis of HCC.Methods The expression of NuSAP1 in 61 cases of HCC and non-cancerous tissues were assessed by RT-PCR,quantitative PCR and immunohistochemistry.The relationship between the expression and the clinicopathological features was studied.Results The levels of mRNA and protein in HCC were higher than the non-cancerous tissues (P＜0.05).On univariate analysis,the expression levels of NuSAP1,mRNA and protein in HCC were significantly associated with TNM classification,lymphatic metastasis,early recurrence,tumor thrombosis and histological differentiation (P＜0.05).Multivariate analysis showed early recurrence was associated with the expression of NuSAP1 protein in HCC (P＜0.05).Overexpression of NuSAP1 protein was correlated with poor outcome of the patients with HCC (x2=15.846,P＜0.001).Conclusions NuSAP1 was overexpressed in hepatocellular carcinoma.Overexpression of NuSAP1 was associated with early postoperative HCC recurrence and bad prognosis.
