Research on bone marrow mesenchymal stem cells in the treatment of severe acute pancreatitis
10.3760/cma.j.issn.1007-8118.2013.09.017
- VernacularTitle:骨髓间充质干细胞治疗重症急性胰腺炎实验研究
- Author:
Yuqiang SHAN
;
Liang LUO
;
Qi XIE
;
Yi WU
;
Zhong JIA
;
Huicheng JIN
;
Guanghua FENG
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Mesenchymal stem cells transplantation;
Interleukins
- From:
Chinese Journal of Hepatobiliary Surgery
2013;19(9):711-715
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of acute inflammatory response and tissue repair when rats accepted transplanted bone marrow mesenchymal stem cells (MSC) in severe acute pancreatitis (SAP).Methods A total of 40 rats were randomly divided into 4 groups which included the normal group (n=10),the model severe acute pancreatitis group (n=10),the transplanted bone marrow mesenchymal stem cells group (n =10),and the combination of bone marrow mesenchymal stem cells and granulocyte colony-stimulating factor (G-CSF) group (n=10).To cure the acute severe pancreatic injury caused by SAP,rats were injected with EDU-labeled MSCs and granulocyte colonystimulating factor (Gr-CSF).The conversion rate of MSCs to pancreatic cells or MSCs to endothelial cells was detected to assess the role of MSCs in tissue regeneration and repair.The expression of amylase,interleukin-6 (IL-6),and interleukin-10 (IL-10) in serum was detected to assess the role of MSCs in an acute inflammatory response.Results The concentrations of amylase and IL-6 were reduced and the concentration of IL-10 was increased in MSC and MSC+G-CSF groups after the onset of SAP.Flow cytometry showed a small amount of MSCs converting to endothelial or pancreatic cells,but the conversion rate was relatively low.Conclusions MSCs can play an important role in the antipre-release of inflammatory mediators,reducing acute immune response to control the acute inflammatory response in SAP.Moreover,MSCs can repair a damaged pancreas by converting into both pancreatic and endothelial cells.
