Fsp27 gene inhibits the proliferation and activation of hepatic stellate cells in vitro

VernacularTitle:脂肪特异性蛋白27基因抑制大鼠肝星状细胞增殖活化
Author: Fuxiang YU ; Caixin SONG ; Zhiwei WU ; Qiandong ZHU ; Qiyu ZHANG
Publication Type:Journal Article
Keywords: Fat-specific protein 27; Genes; Hepatic stellate cells; Proliferation; Activation
From: Chinese Journal of Hepatobiliary Surgery 2013;19(9):701-705
CountryChina
Language:Chinese
Abstract: Objective To investigate the Fsp27 gene's influence on the regulation of hepatic stellate cells (HSCs) in vitro.Methods HSCs were isolated from rat liver,the Fsp27 gene was detected in primary HSCs,and activated HSCs were detected by RT-qPCR.After 72 h of Fsp27 transduction through a lentivirus expressing Fsp27 (pLV-Fsp27),the proliferation of HSCs was tested by the CCK-8 test kit,smooth muscle α-actin (α-SMA) expression of HSCs was tested by Western blot,and the fibrosis-related genes were tested by RT-qPCR.Results The HSCs were isolated and cultured successfully,and the Fsp27 genetic difference between primary and activated HSCs was significant (P＜0.01).After coculture for 72 h,Fsp27 inhibited the proliferation and activation of HSCs (P＜0.05).Fsp27 can enhance expression of the MMP-2 gene and down-regulate expression of the TIMP-1 and TGF-β1 gene in activated HSCs (P＜0.05).Conclusion The Fsp27 gene can inhibit the proliferation and activation of HSCs,regulate the expression of fibrosis-related genes,and may play an important role in maintaining the quiescent phenotype of HSCs.